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Cultured human keratinocytes for optical transmission measurement

✍ Scribed by David Schaaf; Thomas Eurell; Thomas Johnson

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
289 KB
Volume
3
Category
Article
ISSN
1864-063X

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✦ Synopsis

Abstract

The challenges of measuring optical properties of human tissues include the thickness of the sample, homogenization, or crystallization from freezing of the tissue. This investigation demonstrates a method to avoid these problems by growing optically thin samples of human keratinocytes as a substitute for ex vivo epidermis samples. Several methods of growth were investigated. Resulting samples were measured on a spectrophotometer for transmission between 300 nm and 2600 nm. The efficacy of the cell growth was confirmed with histological examination of several cultured keratinocyte samples. Limitations were the requirement to measure samples immediately after removal from the incubation environment, and the absence of the irregular structures of normal skin such as hair and glands. (Β© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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