Culture studies of human pluripotent hemopoietic progenitors
β Scribed by Messner, H. A. ;Fauser, A. A.
- Publisher
- Springer-Verlag
- Year
- 1980
- Weight
- 712 KB
- Volume
- 41
- Category
- Article
- ISSN
- 1432-0584
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Pluripotent (CFU-MIX), erythroid (BFU-E) and granulocyte/macrophage (CFU-GM) progenitor cells were examined in bone marrow (BM) from 23 patients with myelodysplastic syndromes (MDS). Patients were grouped according to the FAB classification: Refractory anemia (RA), n = 3; RA with ring sideroblasts (
Methylcellulose cultures containing mouse marrow cells at low densities and partially purified preparations of erythropoietin and Interleukin-3 were scored after 2 weeks for the presence of macroscopic multilineage colonies (from "primary" CFU-macro GEMM). Whole cultures were then harvested and repl
Several investigators have described hemopoietic colonies expressing multilineage differentiation in culture. We recently identified a class of murine hemopoietic progenitors which form blast cell colonies with very high replating efficiencies. In ordeT to clarify further t h e relationship between