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Culture requirements for the production of protease byAspergillus oryzaein solid state fermentation

โœ Scribed by R. A. Battaglino; M. Huergo; A. M. R. Pilosof; G. B. Bartholomai

Publisher
Springer
Year
1991
Tongue
English
Weight
429 KB
Volume
35
Category
Article
ISSN
1432-0614

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โœฆ Synopsis

A number of culture conditions for protease production by Aspergillus oryzae N R R L 2160 on solid substrates were investigated. The p H of the medium a n d the substrate markedly affected protease production. High protease yield was obtained when the fungus was cultivated for 72-96 h on rice hulls: rice bran (7:3), at an initial p H of 7.0. Maximal protease production was achieved at an initial moisture content of 35-40%, corresponding to a water activity range of 0.982-0.986. Casein and gluten were effective inducers. Polyethylene bags proved to be promising containment systems for solid state cultivation.

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