Solid-state fermentation of soyhull for the production of cellulase

✍ Scribed by Krishna Jha; S.K. Khare; A.P. Gandhi

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 193 KB
• Volume: 54
• Category: Article
• ISSN: 0960-8524
• DOI: 10.1016/0960-8524(95)00154-9

✦ Synopsis

Soyhull, a lignocellulosic material produced in large amounts during soybean processing, was utilized for cellulase production through solid-state fermentation. Of five known fungi, Phanerochaete chrysosporium gave maximum yields of the enzyme. Optimum pH of the substrate and temperature of incubation were 4.0 and 25°C. Under optimized conditions, the addition of urea at 2% (w/w) could enhance the enzyme yields to 74.8 1U/gram dry solids (CMCase) and 29"1 IU/gds (FPase) from the initial values of 27.5 IU/gds (CMCase) and 12.2 IU/gds (FPase). The optimum size of the inoculum was 1.0 x 104 viable spores/gds.