## Several analytical techniques were applied to obtain information about the stability of expression, yield and integrity of monoclonal antibodies (Mabs) produced by hybridoma cells in homogeneous continuous perfusion culture systems. An isotype-specific spot enzyme-linked immunosorbent assay (sp
Cultivation of the hybridoma cell line MN12 in a homogeneous continuous culture system: Effect of culture age
✍ Scribed by José M. Coco-Martin; Dirk E. Martens; Tiny A. M. Velden-de Groot; E. Coen Beuyery
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 533 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0920-9069
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## Abstract The impact of __bcl‐2__ over‐expression on the glycosylation pattern of an antibody produced by a __bcl‐2__ transfected hybridoma cell line (TB/C3.bcl‐2) was investigated in suspension batch, continuous and high cell density culture (Flat hollow fibre, Tecnomouse system). In all culture