Cultivation of mammalian cells on macroporous microcarriers
β Scribed by Thomas J. Nikolai; Wei-Shou Hu
- Book ID
- 103523099
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 703 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0141-0229
No coin nor oath required. For personal study only.
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Cultivation of the new immortalized hepatocyte cell line HepZ was performed with a 1:1 mixture of DMEM and Ham's F12 media containing 5% FCS. The cells were grown in their 40th passage in 100 mL and 1 L volumes in spinner flasks and in a bioreactor, respectively. For the production of adherently gro
## Abstract For the cultivation of mammalian cells on microcarriers a minimum inoculum concentration is required to initiate cell attachment and subsequent cell growth. A critical cell number model has been proposed to elucidate the mechanism of the inoculum requirement. In this model it was hypoth