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Cultivation of human immunodeficiency virus from whole blood: Effect of anticoagulant and inoculum size on virus growth

✍ Scribed by Gary J. Bayliss; William J. Jesson; Philip P. Mortimer; Kenneth A. McLean; Brian A. Evans

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 330 KB
Volume: 31
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890310215

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Human immunodeficiency virus (HIV) was cultivated directly from whole blood treated with anticoagulant by cocultivation with phytohaemagglutinin‐stimulated cord blood lymphocytes. When heparin was used as the anticoagulant, isolation rates were low (10% to 56%, depending on the patient group); but when EDTA was used, isolation rates were much higher (50% to 100%). Culture of whole blood gave results identical to those of culture of separated peripheral mononuclear cells, and in some cases virus could be isolated from as little as 10 μl of unseparated EDTA anticoagulated blood.

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