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Crystallization of the Aα subunit of protein phosphatase 2A

✍ Scribed by Michael O. Skidmore; Gernot Walter; Michael R. Sawaya; Stephen J. Everse; Sean Parkin; Bernhard Rupp; Håkon Hope

Publisher
Cold Spring Harbor Laboratory Press
Year
2008
Tongue
English
Weight
289 KB
Volume
5
Category
Article
ISSN
0961-8368

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✦ Synopsis

Abstract

The Aα subunit of human protein phosphatase 2A forms crystals in space group __P__2~1~ with cell dimensions a = 104.0, b = 174.9, c = 168.2 Å, and β angle = 90.2°. At cryogenic temperatures, the crystals diffracted to a resolution limit of ∼ 3.0 Å. Based on the unit cell dimensions and a calculated molecular mass of 65,277 Da, the Matthews coefficient suggests eight molecules per asymmetric unit. Two native data sets were collected to a nominal resolution of 3.0 Å and merged to provide a set that is 93% complete, with R~svm~ of 9.9%.

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