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Crystallization and Preliminary X-Ray Structure Analysis of a Hyperthermostable Thioltransferase from the ArchaeonPyrococcus furiosus

✍ Scribed by Bin Ren; Gudrun Tibbelin; Donatella de Pascale; Mosè Rossi; Simonetta Bartolucci; Rudolf Ladenstein


Book ID
115633841
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
116 KB
Volume
119
Category
Article
ISSN
1047-8477

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Crystallization and preliminary X-ray cr
✍ David L. Roberts; Jennifer A. Runquist; Henry M. Miziorko; Jung-Ja P. Kim 📂 Article 📅 1995 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 914 KB

## Abstract A recombinant form of __Rhodobacter sphaeroides__ phosphoribulokinase (PRK), expressed in __Escherichia coli__ and isolated by affinity chromatography, was crystallized by the sitting drop vapor diffusion technique using NH~4~H~2~PO~4~ (pH 5.6) as the precipitating agent. PRK crystalliz