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Crystallization and preliminary X-ray diffraction studies of a toxic phospholipase A2 from the venom of Vipera ammodytes meridionalis complexed to a synthetic inhibitor

✍ Scribed by Dessislava Nikolova Georgieva; Wojciech Rypniewski; Markus Perbandt; Mahendra Jain; Nicolay Genov; Christian Betzel

Publisher: Elsevier Science
Year: 2003
Tongue: English
Weight: 86 KB
Volume: 1650
Category: Article
ISSN: 1570-9639
DOI: 10.1016/s1570-9639(03)00210-3

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✦ Synopsis

A toxic phospholipase A(2) (PLA(2)) is isolated from the neurotoxic complex Vipoxin, the major lethal component of the venom of Vipera ammodytes meridionalis. The enzyme is complexed to the synthetic inhibitor elaidoylamide and crystallized. The crystals belong to the space group P2(1)2(1)2(1), with unit cell dimensions a=46.57 A, b=82.68 A, c=119.47 A and beta=90 degrees. Initial diffraction data to 3.3 A resolution are collected.

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