Crystallization and preliminary X-ray cr
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David L. Roberts; Jennifer A. Runquist; Henry M. Miziorko; Jung-Ja P. Kim
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Article
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1995
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Cold Spring Harbor Laboratory Press
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English
β 914 KB
## Abstract A recombinant form of __Rhodobacter sphaeroides__ phosphoribulokinase (PRK), expressed in __Escherichia coli__ and isolated by affinity chromatography, was crystallized by the sitting drop vapor diffusion technique using NH~4~H~2~PO~4~ (pH 5.6) as the precipitating agent. PRK crystalliz