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Crystallization and preliminary X-ray analysis of the C-terminal WRKY domain of Arabidopsis thaliana WRKY1 transcription factor

✍ Scribed by Ming-Rui Duan; Hui Ren; Peng Mao; Chun-Hong Wei; Yu-He Liang; Yi Li; Xiao-Dong Su

Publisher: Elsevier Science
Year: 2005
Tongue: English
Weight: 105 KB
Volume: 1750
Category: Article
ISSN: 1570-9639
DOI: 10.1016/j.bbapap.2005.03.013

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✦ Synopsis

The C-terminal WRKY domain of Arabidopsis thaliana WRKY1 protein, a transcription factor, was cloned and expressed. The expressed protein was then purified and crystallized. The preliminary X-ray analysis was undertaken. The crystal diffracted to 2.50 A ˚resolution inhouse and belongs to space group P2 1 with unit-cell parameters a = 64.

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