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Crystallization and preliminary X-ray analysis of outer membrane phospholipase A from Escherichia coli

✍ Scribed by Mieke Blaauw; Niek Dekker; Hubertus M. Verheij; Kor H. Kalk; Bauke W. Dijkstra

Book ID: 115931340
Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 266 KB
Volume: 373
Category: Article
ISSN: 0014-5793
DOI: 10.1016/0014-5793(95)01002-v

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## Peptidyl-tRNA hydrolase from Escherichia coli, a monomer of 21 kDa, was overexpressed from its cloned gene pth and crystallized by using polyethylene glycol as precipitant. The crystals are orthorhombic and have unit cell parameters a 5 47.24 Å, b 5 63.59 Å, and c 5 62.57 Å. They belong to spac