✦ LIBER ✦

Crystallization and preliminary structural studies of neurotrophin-3

✍ Scribed by Jennifer A. Kelly; Elizabeth Singer; Timothy D. Osslund; Todd O. Yeates

Publisher: Cold Spring Harbor Laboratory Press
Year: 2008
Tongue: English
Weight: 252 KB
Volume: 3
Category: Article
ISSN: 0961-8368
DOI: 10.1002/pro.5560030614

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Neurotrophin‐3 (NT‐3) has been crystallized in 2 forms. Orthorhombic crystals, space group P2~1~2~1~2, diffracted to 2.8 Å and have cell dimensions a = 39.1 Å, b = 54.0 Å, and c = 65.5 Å. The second form is space group P4~3~2~1~2, with cell dimensions a = b = 67.1 Å, and c = 107.9 Å. The tetragonal crystals diffract to 2.8 Å at room temperature and 2.5 Å at −100 °C. The unit cell dimensions change significantly upon freezing, a = b = 66.1 Å, and c = 102.8 Å. Phases for the orthorhombic form were obtained by molecular replacement using nerve growth factor as the search model. A partially refined model of the NT‐3 dimer (75% complete) was then oriented and positioned in the tetragonal cell.

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