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Crystallization and Preliminary Diffraction Studies of Escherichia coli Lysyl-tRNA Synthetase (LysU)

โœ Scribed by Silvia Onesti; Maria-Elena Theoclitou; E. Pernilla; L. Wittung; Andrew D. Miller; Pierre Plateau; Sylvain Blanquet; Peter Brick


Book ID
115626870
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
187 KB
Volume
243
Category
Article
ISSN
0022-2836

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## Peptidyl-tRNA hydrolase from Escherichia coli, a monomer of 21 kDa, was overexpressed from its cloned gene pth and crystallized by using polyethylene glycol as precipitant. The crystals are orthorhombic and have unit cell parameters a 5 47.24 ร…, b 5 63.59 ร…, and c 5 62.57 ร…. They belong to spac