Crystalline degradation products of vancomycin as chiral stationary phase in microcolumn liquid chromatography

✍ Scribed by Alireza Ghassempour; Reza Alizadeh; Nahid Mashkori Najafi; Ahmad Karami; Andreas Römpp; Bernhard Spengler; Hassan Y. Aboul-Enein

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 682 KB
• Volume: 31
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200800185

✦ Synopsis

Abstract

The ability of crystalline degradation products (CDPs) of vancomycin as a chiral stationary phase was reported in a previous study for enantioselective separation of drugs, amino acids and agrochemical toxins by conventional LC column (250×4.6 mm). In this work, the potential of CDP of vancomycin for the enantiomeric separation in micro‐LC (200×1 mm) has been studied. The obtained separation results are better than in our previous study with conventional LC columns. The enantiomers of D,L‐phenylalanine, D,L‐alanine, methyldopa, atropine and propranolol were used for this evaluation. Experiments have been carried out in a stainless steel tube that was packed with chiral silica particles of 3 and 12 μm diameters. Also, three different ratios of 3 and 12 μm silica particles were used for packing material of chiral columns and the effect on aspect ratio and resolving powers was compared.