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Crystal structure of proflavine, a DNA binding agent

✍ Scribed by NEIDLE, S.; JONES, T. A.

Book ID
109694136
Publisher
Nature Publishing Group
Year
1975
Tongue
English
Weight
236 KB
Volume
253
Category
Article
ISSN
0028-0836

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## Abstract Proflavine binding may be used as a probe of the environment and interactions of DNA. In this paper we report the effects of the divalent cations Mg^++^ and putrescine and the trivalent cation spermidine on the proflavine–Na DNA binding equilibrium. Difference spectroscopy at 430 nm was

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## Abstract We have measured the binding equilibria of proflavin to T2L bacteriophage, in both β€œslow” and β€œfast” sedimenting forms, and to free T2L DNA. Measurements were carried out by difference spectroscopy at 430 nm at temperatures from 13 to 43Β°C and at pH 5.6 and 7.6. We found no significant