Cryopreservation ofin vitro-grown apical
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T. Matsumoto; A. Sakai; K. Yamada
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Article
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1994
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Springer
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English
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In vitro-grown apical meristems of wasabi (Wasabia japonica Matsumura) were successfully cryopreserved by vitrification. Excised apical meristems precultured on solidified M S medium containing 0.3M sucrose at 20ยฐC for 1 day were loaded with a mixture of 2M glycerol and 0.4M sucrose for 20 min at 25