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Cryopreservation of in vitro-grown apical meristems of Lilium by droplet-vitrification

โœ Scribed by X.-L. Chen; J.-H. Li; X. Xin; Z.-E. Zhang; P.-P. Xin; X.-X. Lu

Book ID
113882361
Publisher
Africa Journals Online
Year
2011
Tongue
English
Weight
591 KB
Volume
77
Category
Article
ISSN
1727-9321

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Cryopreservation ofin vitro-grown apical
Cryopreservation ofin vitro-grown apical meristems of wasabi (Wasabia japonica) by vitrification and subsequent high plant regeneration
โœ T. Matsumoto; A. Sakai; K. Yamada ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Springer ๐ŸŒ English โš– 711 KB

In vitro-grown apical meristems of wasabi (Wasabia japonica Matsumura) were successfully cryopreserved by vitrification. Excised apical meristems precultured on solidified M S medium containing 0.3M sucrose at 20ยฐC for 1 day were loaded with a mixture of 2M glycerol and 0.4M sucrose for 20 min at 25