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Cryopreservation of Grapevine (Vitisspp.) Embryogenic Cell Suspensions by Encapsulation–Vitrification

✍ Scribed by Qiaochun Wang; Munir Mawassi; Nachman Sahar; Ping Li; Colova-Tsolova Violeta; Ron Gafny; Ilan Sela; Edna Tanne; Avihai Perl

Book ID
111613333
Publisher
Springer
Year
2004
Tongue
English
Weight
246 KB
Volume
77
Category
Article
ISSN
0167-6857

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✍ Chun-Nong Huang; Jun-Hui Wang; Qiu-Sheng Yan; Xue-Qin Zhang; Qin-Feng Yan 📂 Article 📅 1995 🏛 Springer 🌐 English ⚖ 767 KB

Rice cells were precultured for 10 d in medium containing 60 g/L sucrose and subsequently for 1 d in medium supplemented with 0. 4 M sorbitol. After loading with 25%PVS2 at 22°C for 10 min and dehydration in 100%PVS2 at 0°C for 7. 5 min,they were plunged into liquid nitrogen directly. Survival was 4