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Crosslinking of substrates occurs exclusively to the p66 subunit of heterodimeric HIV-1 reverse transcriptase

✍ Scribed by Nancy Cheng; George R. Painter; Phillip A. Furman


Book ID
115764337
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
740 KB
Volume
174
Category
Article
ISSN
0006-291X

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Preparations of recombinant HIV-1 p66 an
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Recombinant HIV-1 p66 (rp66, a subunit of reverse transcriptase (RT), a heterodimer of p66 and p51) was produced in Escherichia coli in three different ways. First, rp66 was produced as a part of the fusion protein of lacZ protein and HIV-1 pol protein consisting of three components: protease (p10),