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Cross-sectional analysis of germline BRCA1 and BRCA2 mutations in Japanese patients suspected to have hereditary breast/ovarian cancer

โœ Scribed by Kokichi Sugano; Seigo Nakamura; Jiro Ando; Shin Takayama; Hiroyuki Kamata; Isao Sekiguchi; Megumi Ubukata; Tetsuro Kodama; Masami Arai; Fujio Kasumi; Yasuo Hirai; Tadashi Ikeda; Hiromitsu Jinno; Masaki Kitajima; Daisuke Aoki; Akira Hirasawa; Yuko Takeda; Kumiko Yazaki; Takashi Fukutomi; Takayuki Kinoshita; Ryuichiro Tsunematsu; Teruhiko Yoshida; Masako Izumi; Shino Umezawa; Hiroshi Yagata; Hiroko Komatsu; Naoko Arimori; Noriko Matoba; Nobuhisa Gondo; Shiro Yokoyama; Yoshio Miki


Book ID
108584324
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
293 KB
Volume
99
Category
Article
ISSN
1347-9032

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Germline mutations in the BRCA1 and BRCA
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In this study we genotyped Turkish breast/ovarian cancer patients for BRCA1/BRCA2 mutations: protein truncation test (PTT) for exon 11 BRCA1 of and, multiplex PCR and denaturing gradient gel electrophoresis (DGGE) for BRCA2, complemented by DNA sequencing. In addition, a modified restriction assay w