Critical thin-layer chromatography as a novel method for the determination of the molecular weight and compositional inhomogeneity of block copolymers
β Scribed by B.G. Belenkii; E.S. Gankina; V.N. Zgonnik; I.I. Malchova; E.U. Melenevskaya
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 582 KB
- Volume
- 609
- Category
- Article
- ISSN
- 1873-3778
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Saccharin is extracted from the acidified liquid food with chloroform. This solvent is evaporated ; the residue is dissolved in ethyl acetate and applied on a silica gel GF,,, plate. After chromatographic separation, the saccharin band is scraped off and extracted with 1 % sodium carbonate solution.
Elution characteristics of collagen-derived polypeptides and of globular proteins were compared under identical experimental conditions with agarose gels. This comparison permitted calculation of the hydrodynamic radii of collagen polypeptide chains of different molecular weight, and these radii wer