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CRISPR: Methods and Protocols

✍ Scribed by Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran (eds.)


Publisher
Humana Press
Year
2015
Tongue
English
Leaves
371
Series
Methods in Molecular Biology 1311
Edition
1
Category
Library

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✦ Synopsis


This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge,CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.

✦ Table of Contents


Front Matter....Pages i-xiv
Back Matter....Pages 1-21
....Pages 23-33

✦ Subjects


Gene Expression


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