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Covalently closed minicircular DNA in Micrococcus lysodeikticus

โœ Scribed by Chong Sung Lee; Norman Davidson


Book ID
118850545
Publisher
Elsevier Science
Year
1968
Tongue
English
Weight
726 KB
Volume
32
Category
Article
ISSN
0006-291X

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Quantitative binding of covalently close
โœ Joel Bresser; David Gillespie ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 696 KB

A procedure for quantitativel y binding covalently closed circular DNA to nitrocellulose is detailed. The procedure involves making DNA saturated with respect to NaI at 25ยฐC, denaturing the DNA by heating to 80-100 cC, then passing the DNA-NaI solution through a nitrocellulose membrane at 50-60ยฐC. F