Covalently closed circles of adenovirus 5 DNA
β Scribed by Ruben, Martha; Bacchetti, Silvia; Graham, Frank
- Book ID
- 109729626
- Publisher
- Nature Publishing Group
- Year
- 1983
- Tongue
- English
- Weight
- 511 KB
- Volume
- 301
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/301172a0
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A procedure for quantitativel y binding covalently closed circular DNA to nitrocellulose is detailed. The procedure involves making DNA saturated with respect to NaI at 25Β°C, denaturing the DNA by heating to 80-100 cC, then passing the DNA-NaI solution through a nitrocellulose membrane at 50-60Β°C. F
This study examined a signal amplification assay, the Invader assay, for the quantitation of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) in liver biopsies and sera. DNA was extracted from liver biopsy and serum samples were collected from 16 hepatitis B e antigen (HBeAg)-positive