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Covalently Bound Flavin in d-6-Hydroxynicotine Oxidase from Arthrobacter oxidans : Purification and Properties of d-6-Hydroxynicotine Oxidase

✍ Scribed by Margarete Brühmüller; Hanns Möhler; Karl Decker


Book ID
115113250
Publisher
John Wiley and Sons
Year
1972
Tongue
English
Weight
826 KB
Volume
29
Category
Article
ISSN
1432-1327

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The 160 kb plasmid pAO1 from Arthrobacter oxidans (Brandsch and Decker 1984) was subcloned in Escherichia coli with the aid of the plasmid vectors pUR222 and pBR322. Screening of the recombinant clones for enzyme activity revealed that the flavoenzyme 6-hydroxy-D-nicotine oxidase (6-HDNO), one of th