Coupling of histone mRNA levels to radioresistant DNA synthesis in ataxia-telangiectasia cells

✍ Scribed by Martin F. Lavin; Jane Houldsworth; Sharad Kumar; Janet L. Stein; Gary S. Stein

• Book ID: 104673505
• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 658 KB
• Volume: 73
• Category: Article
• ISSN: 0300-8177
• DOI: 10.1007/bf00229375

✦ Synopsis

Cloned genomic DNA for human histone H1, H3 and H4 genes has been used to determine the effects of gamma-radiation on histone mRNA levels and synthesis in ataxia-telangiectasia cells. Synthesis of histone mRNA was determined in cells synchronized with aphidicolin. Effects of irradiation on DNA synthesis and passage through S phase were also monitored. Irradiation was found to slow the passage of control cells through the cell cycle but had no effect on progression of ataxia-telangiectasia cells. H1 and core histone mRNA synthesis was inhibited by radiation in two control cell lines after release from aphidicolin block. No inhibition was observed in one ataxia-telangiectasia cell line and a small degree of inhibition in a second. An increased level of mRNA was observed in both irradiated control and ataxia-telangiectasia cells at 5-7 h post-irradiation compared to unirradiated cells. Similar results were obtained in log phase cells. These results demonstrate that histone mRNA synthesis is radioresistant in ataxia-telangiectasia cells and is coupled to radioresistant DNA synthesis in these cells.