𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Coupled enzyme systems: Exploring coupled assays with students

✍ Scribed by Marie Louise Uhr

Book ID
103702717
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
353 KB
Volume
18
Category
Article
ISSN
0307-4412

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Optimizing coupled enzyme assays
Optimizing coupled enzyme assays
✍ W.W. Cleland πŸ“‚ Article πŸ“… 1979 πŸ› Elsevier Science 🌐 English βš– 295 KB

Equations are given for minimizing the cost and calculating the maximum practical rates for coupled assays when two or more coupling enzymes are involved, and a lag of a predetermined size can be tolerated. The lag caused by obligatory mutarotation of an intermediate is also calculated.

Enzyme-Coupled Assays for Proteases
Enzyme-Coupled Assays for Proteases
✍ Brian E. Cathers; John V. Schloss πŸ“‚ Article πŸ“… 1996 πŸ› Elsevier Science 🌐 English βš– 104 KB
Optimizing enzyme assays with one or two
Optimizing enzyme assays with one or two coupling enzymes
✍ F. GarcΓ­a-Carmona; F. GarcΓ­a-CΓ‘novas; J.A. Lozano πŸ“‚ Article πŸ“… 1981 πŸ› Elsevier Science 🌐 English βš– 355 KB

The cost of assays using one or two coupling enzymes is optimized by using equations to calculate the minimum amount(s) of enzyme(s) which should be used to obtain a given time (r& in which 99% of the rate V, of the first reaction is obtained. Using two coupling enzymes and given a value of rae, the