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Countercurrent chromatography with a new horizontal flow-through coil planet centrifuge

✍ Scribed by Yoichiro Ito

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 663 KB
Volume: 100
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90230-6

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✦ Synopsis

A versatile horizontal flow-through coil planet centrifuge has been introduced for performing countercurrent chromatography. The apparatus carries a pair of coiled separation columns. Both of these allow continuous elution simultaneously without the use of rotating seals. One column enables preparative-scale separations and the other, analytical-scale separations, both with a high partition efficiency comparable to that obtained in liquid chromatography but without the complications arising from the use of solid supports. Capability of the apparatus was demonstrated on separation of dinitrophenyl amino acids using a two-phase solvent system composed of CHCl&HsCOOH/O.l N HCl at a 2/2/l volume ratio.

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