Coulometric Titration of Hydroxyurea

✍ Scribed by Vladimir Dosortzev; Emilia Kirowa-Eisner

• Publisher: Elsevier Science
• Year: 1997
• Tongue: English
• Weight: 165 KB
• Volume: 57
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1006/mchj.1997.1507

✦ Synopsis

Hydroxyurea, HXU, is quantitatively determined by electrochemically generated bromine. The overall reaction between HXU and Br 2 is studied and determined to be NH 2 CONHOH / Br 2 / 1 2 H 2 O r HBr / NH 4 Br / CO 2 / 1 2 N 2 O. The titrations are performed in the monotonic consecutive mode with the use of a nonisolated counter electrode. The end point is detected amperometrically (with a gold or platinum indicating electrode) or potentiometrically (with a gold indicating electrode). The chemical composition of the solution is 0.4 M KBr in acetate buffer. Concentrations of HXU in the range from 5 mM to 0.1 mM are determined with a standard deviation of 1-2%, respectively.