Coulometric titration of proteins with electrogenerated hypobromite

✍ Scribed by Gary D. Christian

• Publisher: Elsevier Science
• Year: 1966
• Tongue: English
• Weight: 517 KB
• Volume: 14
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(66)90126-6

✦ Synopsis

The coulometric determination of urea nitrogen (1) was recently reported. Ammonia was titrated with electrogenerated hypobromite following urease hydrolysis of the sample. In this study, it was noted that native protein in serum samples resulted in abnormally high titration blanks, i.e., the protein reacted with the generated hypobromite.

The titration value of the serum proteins appeared to be fairly constant for a given set of conditions. Thus, it seemed possible to develop a rapid, direct coulometric titration of proteins. A number of variables have been investigated, including sample size, solution volume, generating rate, the nature of the protein, the age of serum samples, and the effect of freezing.

Csontos has described the titration of serum proteins with potassium permanganat,e (2).