## Abstract The steady‐state metabolic parameters for a hybridoma cell line have been determined in continuous suspension–perfusion culture over a wide range of perfusion rates and cell bleed rates. Significant increases in viable cell concentrations and volumetric productivities were achieved at h
Correlation between steady-state cell concentration and cell death of hybridoma cultures in chemostat
✍ Scribed by Yuan-Kun Lee; Peng-Kang Yap; Ai-Peng Teoh
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 676 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0006-3592
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Experimental data from six hybridoma cell lines grown under diverse experimental conditions in both normal continuous and perfusion cultures are analyzed with respect to the significance of nutrients and products in determining the growth and death rates of cells and with respect to their mathematic
## Abstract Constant intracellular concentration of both adenosine 3′,5′ cyclic monophosphate (cyclic AMP) and guanosine 3′,5′ cyclic monophosphate (cyclic GMP) were obtained when mouse leukemia L1210 cells were cultivated under steady‐state conditions in the chemostat. When L1210 cells were mainta