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Correction: Sequence-Specific Cleavage of Single-Stranded DNA : Oligodeoxynucleotide-EDTA.Fe(II)


Book ID
123631894
Publisher
National Academy of Sciences
Year
1985
Tongue
English
Weight
171 KB
Volume
82
Category
Article
ISSN
0027-8424

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A simple procedure for the precise assembly of functional DNA sequences from overlapping fragments is described. The fragments to be joined are cloned in tandem in the proper relative orientation into a vector from which single-stranded DNA copies can be obtained. Single-stranded DNA is cut by a res