We describe frequency-domain measurements of the time-dependent intensity decays of pbis [2-(5-phenyloxazolyl) Ibenzene (PCJPOP) when collisionally quenched by carbon tetrabromide ( CBr,). Tbe frequency-domain data reveal the existence of a quenching rate constant which depends exponentially on the
Correction for contaminant fluorescence in frequency-domain fluorometry
โ Scribed by Joseph R. Lakowicz; Ranjith Jayaweera; Nanda Joshi; Iganzy Gryczynski
- Book ID
- 102987575
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 663 KB
- Volume
- 160
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Gated detection is often used in time-domain measurements of long-lived fluorophores for suppression of interfering short-lived autofluorescence. However, no direct method has been available for gated detection and background suppression when using frequency-domain fluorometry. We describe a direct
Ouenching of 1,2\_benzanthracene (BA) by CBr4 was studied by frequency-domain fluorometry. The frequency response of the BA emission could not be fit using the Smoluchowski decay law for transient effects in quenching, exp( -t/s -2bt "z). However, the data were consistent with the expression for the