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Coordinated regulation of endothelial nitric oxide synthase activity by phosphorylation and subcellular localization

✍ Scribed by Yong Chool Boo; Hyo Jung Kim; Hannah Song; David Fulton; William Sessa; Hanjoong Jo

Book ID: 113629970
Publisher: Elsevier Science
Year: 2006
Tongue: English
Weight: 526 KB
Volume: 41
Category: Article
ISSN: 0891-5849
DOI: 10.1016/j.freeradbiomed.2006.03.024

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1179A eNOS and HA-Akt overnight. 36 h after transfection, we placed cells into dialysed serum-replete, phosphate-free Dulbecco's minimum essential medium supplemented with 80 Ci per ml of 32 P orthophosphoric acid for 3 h. Some cells were pretreated with wortmannin (500 nM) in the phosphate-free med