Cooperative Elements in Protein Folding Monitored by Electrospray Ionization Mass Spectrometry
β Scribed by Hooke, Shaun D.; Eyles, Stephen J.; Miranker, Andrew; Radford, Sheena E.; Robinson, Carol V.; Dobson, Christopher M.
- Book ID
- 126072675
- Publisher
- American Chemical Society
- Year
- 1995
- Tongue
- English
- Weight
- 300 KB
- Volume
- 117
- Category
- Article
- ISSN
- 0002-7863
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract An electrospray ionisation (ESI) mass spectrometric method for the determination of the equilibrium constant and free energy (Ξ__G__) of protein unfolding was used to monitor the denaturation process at different pH of three metalloβproteins, i.e. wildβtype copper azurin, zinc azurin an
Crude mixtures of difficult tetrapeptide cyclization reactions were analyzed by electrospray ionization mass spectrometry to optimize the production of cyclomonomer versus cyclodimer. The cationization phenomenon enabled the differentiation between monoprotonated and multiply charged ions of the sam