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Conversion of gibberellin A20to gibberellins A1and A5in a cell-free system fromPhaseolus vulgaris

✍ Scribed by Yuji Kamiya; Masahiro Takahashi; Nobutaka Takahashi; Jan E. Graebe

Publisher: Springer-Verlag
Year: 1984
Tongue: English
Weight: 515 KB
Volume: 162
Category: Article
ISSN: 0032-0935
DOI: 10.1007/bf00410212

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✦ Synopsis

The soluble fraction of a cell-free system from immature seeds of Phaseolus vulgaris L. converts gibberellin A20 (GA20) to GA s and GA 5.

It does however not metabolize GA1 and GA29

to GA 5, showing that in this system GA2o is converted directly to GA 5. The steps from GAzo to GA s (3-hydroxylation) and from GA20 to GA 5 (A 2 double-bond formation) require oxygen, Fe 2 + and ~-ketoglutarate, and are stimulated by ascotbate. The enzymes catalyzing these conversions have properties similar to those of GA oxidases found in Cucurbita maxima and Pisum sativum.

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