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Conversion of carbamoyl phosphate to hydroxyurea: An Assay for Carbamoylphosphate Synthetase

โœ Scribed by Rodney L. Levine; Norman Kretchmer


Book ID
107711168
Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
685 KB
Volume
42
Category
Article
ISSN
0003-2697

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An indirect, competitive enzyme-linked immunosorbent assay for the quantitation of carbamoyl-phosphate synthetase I (ammonia) in rat liver has been developed. Homogenization of the liver in 1% sodium deoxycholate is used for complete solubilization of the enzyme. The detergent does not interfere wit