Conventional and microwave-assisted SPPS approach: a comparative synthesis of PTHrP(1–34)NH2

✍ Scribed by Fabio Rizzolo; Chiara Testa; Duccio Lambardi; Michael Chorev; Mario Chelli; Paolo Rovero; Anna Maria Papini

• Book ID: 105360022
• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 242 KB
• Volume: 17
• Category: Article
• ISSN: 1075-2617
• DOI: 10.1002/psc.1395

✦ Synopsis

Abstract

Attracted by the possibility to optimize time and yield of the synthesis of difficult peptide sequences by MW irradiation, we compared Fmoc/tBu MW‐assisted SPPS of 1–34 N‐terminal fragment of parathyroid hormone‐related peptide (PTHrP) with its conventional SPPS carried out at RT. MWs were applied in both coupling and deprotection steps of SPPS protocol. During the stepwise elongation of the resin‐bound peptide, monitoring was conducted by performing MW‐assisted mini‐cleavages and analyzing them by UPLC‐ESI‐MS. Identification of some deletion sequences was helpful to recognize critical couplings and as such helped to guide the introduction of MW irradiations to these stages. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd.