Convenient Preparation of Biodegradable PEI-Containing Polymers as Non-Viral Vectors for Gene Transfection

✍ Scribed by Fu-Wei Huang; Jun Feng; Jun Nie; Si-Xue Cheng; Xian-Zheng Zhang; Ren-Xi Zhuo

• Book ID: 102934343
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 859 KB
• Volume: 9
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.200900187

✦ Synopsis

Abstract

Poly(L‐succinimide)‐graft‐polyethylenimines (PSPs) were prepared as non‐viral vectors for gene transfection. Branched polyethylenimine ($\overline M _{\rm w} $ = 800, PEI800) was grafted to poly(L‐succinimide) (PSI) in a one‐step reaction with no catalyst. Gel retardation assay showed that the mobility of PSP/pDNA complexes was completely retarded at the low N/P ratio of 0.42. In vitro transfection experiments showed that, at N/P ratio of 0.84, PSPs can reach the highest transfection level with ten‐fold enhancement in 293T cells and five‐fold enhancement in HeLa cells as compared with PEI25k ($\overline M _{\rm w} $ = 25 000). Fluorescent confocal microscopy showed that pGL‐3 plasmids condensed by PSPs could be effectively transported into the nuclei of HeLa cells. Significantly reduced cytotoxicity of polymers was observed towards 293T and HeLa cells, with the 50% inhibition concentration of PSPs being almost four times higher than that of PEI25k.

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