Convenient method for sex reversal in a freshwater teleost, the medaka

✍ Scribed by Iwamatsu, Takashi

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 93 KB
• Volume: 283
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/(sici)1097-010x(19990201)283:2<210::aid-jez12>3.0.co;2-f

✦ Synopsis

In order to analyze the mechanism of sex differentiation, fertilized eggs of Oryzias latipes with sex-linked R (orange-red) and r (white) phenotypes were exposed to estradiol-17β at various concentrations until hatching, and fry were reared on a normal diet up to sexual maturity. When embryos 0-6 days after fertilization were immersed in saline containing estradiol-17β at 1 µg/ml for only 4-10 days until hatching, the sex reversal of genotypic males to females was induced. The majority of the sex-reversed females (X r Y R ) were fertile, as indicated by the presence of vitellogenetic oocytes in their ovaries. These results show that steroid treatment during embryonic stages is a very convenient method for sex reversal in the medaka.