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Convenient method for preparation and purification of nicotinamide mononucleotide analogs

✍ Scribed by Werner Hensel; Dagmar Rakow; Wolfram Christ

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
525 KB
Volume
68
Category
Article
ISSN
0003-2697

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✦ Synopsis

Nucleotide pyrophosphatase from Crotalus adamanteus venom cleaves the pyrophosphate linkage of NADP +, thio-NADP +, 3-AcPyr-ADP +, seleno-NADP +, thio-NAD +, 3-AcPyr-AD + and 3-OHCPyr-AD +.

  1. The nicotinamide mononucleotide (NMN) analogs can be obtained from reaction mixtures in good yield and high purity by chromatography on Whatman DE 52 cellulose.

  2. The uv spectra and specific extinction coefficients of the NMN analogs were almost identical with those of the corresponding 1-methylpyridinium derivatives.

  3. Thio-NMN, 3-AcPyr-MN and 3-OHCPyr-MN can be condensed with AMP to the corresponding NAD + analogs using dicyclohexylcarbodiimide.

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(4R)-Deuterated-reduced nicotinamide adenine dinucleotide phosphate, \((4 R)-\left[{ }^{2} H\right] N A D P H\), was prepared by reduction of NADP \({ }^{+}\)using an NADP \({ }^{+}\)-dependent alcohol dehydrogenase (EC 1.1 .1 .2 ) from Thermoanaerobium brockii and isopropanol- \(d_{8}\) as substrat

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