## Abstract Efficient and economic recovery of immunoglobulins (Igs) from complex biological fluids such as serum, cell culture supernatant or fermentation cell lysate or supernatant, represents a substantial challenge in biotechnology. Methods such as protein A affinity chromatography and anion ex
Controlled Production of Emulsions Using a Crossflow Membrane
โ Scribed by Shuangjiu Peng; Richard A. Williams
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 173 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0934-0866
No coin nor oath required. For personal study only.
โฆ Synopsis
A method for manufacturing emulsions -crossflow membrane emulsification has been studied. This involves the formation of emulsions by breaking up the discontinuous phase into droplets in a controlled manner without the use of turbulent eddies. This is achieved by passing the discontinuous phase through a suitable microporous medium and injecting the droplets so formed directly into a moving continuous phase. This paper presents two examples of experimental data for droplet formation using a single pore (capillary tube) and a membrane tube. A high-speed video camera (up to 1000 frames per second) was used to record the formation of droplet from a single pore and thus measure droplet growth and the detachment processes as a function of process parameters such as transmembrane pressure drop, membrane pore size, continuous phase crossflow velocity etc. Real emulsions were prepared using a membrane tube.
๐ SIMILAR VOLUMES
Data analysis and graphical examination of the results were performed on the PROPHET computer system, a specialized resource developed by the Chemical/Biological Information Handling Program of the National Institutes of Health (43). We wish to thank Ms. Deborah J. Johnson and Mr. Richard Wagner fo