Controlled band dispersion for quantitative binding determination and analysis with electrospray ionization-mass spectrometry

✍ Scribed by Kevin A. Schug; Carlos Serrano; Petr Fryčák

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 772 KB
• Volume: 29
• Category: Article
• ISSN: 0277-7037
• DOI: 10.1002/mas.20267

✦ Synopsis

Abstract

This review discusses recent emerging techniques that have been used to couple flow‐injection analysis (FIA) and electrospray ionization‐mass spectrometry (ESI‐MS) for the quantitation of noncovalent binding interactions. Focus is placed predominantly on two such methods. Diffusion‐based measurements, developed by Konermann and co‐workers, uses controlled‐band dispersion prior to ESI‐MS to determine diffusion constants and binding constants based on the temporal variation of ligand signal measured in the mass spectrum (an indirect technique). Dynamic titration, developed by Schug and co‐workers, is a direct method, where a temporal compositional gradient of a guest molecule is induced in the presence of host in solution to monitor the concentration dependence of complex formation as a function of observed complex ion abundance after ESI‐MS. Further discussion places these techniques in the context of a variety of other direct and indirect ESI‐MS‐based binding determination methods, and highlights advantages, disadvantages, and practical considerations for their proper use to investigate a broad range of macromolecular and small‐molecule interaction systems. © 2009 Wiley Periodicals, Inc., Mass Spec Rev 29:806–829, 2010