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Determination of zinc to beta-peptide binding constants with electrospray ionization mass spectrometry

✍ Scribed by Arno Wortmann; Francesco Rossi; Gerald Lelais; Renato Zenobi

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 173 KB
Volume: 40
Category: Article
ISSN: 1076-5174
DOI: 10.1002/jms.852

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✦ Synopsis

Abstract

We present an improvement of the titration method for binding constant determination with electrospray ionization (ESI) mass spectrometry that is unaffected by differences in ESI response of measured species in solution. The method consists of a calibration and titration, both using an internal standard that allows relative quantitation. This avoids artifacts such as a decrease in overall signal intensity with increasing ligand concentrations, rendering this approach more reliable and meaningful than direct evaluation of ESI peak intensities. We demonstrate the de novo binding constant determination of novel zinc binding beta‐peptides, which have been synthesized with the goal of creating secondary structures stabilized by metal complexation. Copyright © 2005 John Wiley & Sons, Ltd.

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