Control of Transcription of the Repressor Gene in Bacteriophage Lambda
โ Scribed by Stephen F. Heinemann and Willard G. Spiegelman
- Book ID
- 123649999
- Publisher
- National Academy of Sciences
- Year
- 1970
- Tongue
- English
- Weight
- 306 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/60424
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The high-affinity mutant cI gene of lambda cIha (Nag et al. 1984) was cloned in the multicopy plasmid pBR322. In the resulting plasmid, pMD 102, a lacUV5 promoter was inserted giving the lacUV5-cIha fusion plasmid pMD 205. Bacteria carrying pMD 102 and pMD 205 contain 2.5 and 15 times, respectively,
By mutagenizing a 2cIts (2ci857) lysogen, a 2 mutant has been isolated with a wild-type phenotype. This mutant phage lysogenizes with low efficiency and produces a low burst. Though the initial rates of repressor synthesis in Escherichia coli after infection with wild-type and mutant 2 are the same,