𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Control of mitotic activity in Wolffian lens regeneration

✍ Scribed by Yamada, Tuneo ;Roesel, Marion

Publisher
John Wiley and Sons
Year
1971
Tongue
English
Weight
754 KB
Volume
177
Category
Article
ISSN
0022-104X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The total numbers of mitotic cells in the iris epithelium, ciliary epithelium, and ora serrata were counted at various time intervals after lentectomy of adult newts, Notophthalmus (Triturus) viridescens, in bleached serial sections. In some cases colchicine was injected for the purpose of accumulating mitotic figures.

In the iris epithelium, which normally shows no mitotic cells, a wave of mitoses is induced by lentectomy. The wave starts on day 4 and has its peak period during days 7 to 15, when a portion of the iris epithelial cells depigment and form the lens vesicle. In the iris epithelium the wave subsides after formation of the regenerated lens and is gone by day 30. On the other hand, in the regenerated lens the mitotic activity is retained in the lens epithelium. The colchicine treatment has revealed an extremely small number of iris epithelial cells going into mitoses within one day after lentectomy. However, the same treatment failed to demonstrate any mitotic cells on days 0, 2, and 3.

Mitotic activity is also induced in the ciliary epithelium, which does not take part in formation of the lens regenerate. This induction is much lower in intensity than that in the iris epithelium.

The ora serrata area has a low level of mitotic activity in the normal condition, which is elevated to a high level after day 20. Significance of these observations for the mechanism of lens regeneration is discussed.

Estimation of the total cell number of iris and ciliary epithelia during the first 15 days after lentectomy indicated an increase in the iris epithelium, which can be accounted for by the number of mitoses counted during the corresponding period in the same tissue.

Some data on mitosis in the normal and regenerated lenses are also presented.

πŸ“œ SIMILAR VOLUMES

Activation of ribosomal RNA synthesis in
Activation of ribosomal RNA synthesis in initiation of Wolffian lens regeneration
✍ Reese, David H. ;Puccia, Egidio ;Yamada, Tuneo πŸ“‚ Article πŸ“… 1969 πŸ› John Wiley and Sons 🌐 English βš– 748 KB

Tissue engaged in the transformation of iris into lens, triggered by lens removal in the adult newt (Triturus viridescens), was labeled in vitro with 14curidine, and the sedimentation profiles of RNA synthestized by the tissue were studied in sucrose density gradients. The radioactivity in the 18s a

Pituitary enhancement of wolffian lens r
Pituitary enhancement of wolffian lens regeneration in vitro: Spatial and temporal requirements
✍ Connelly, Thomas G. πŸ“‚ Article πŸ“… 1977 πŸ› John Wiley and Sons 🌐 English βš– 621 KB

## Abstract When irido‐corneal complexes (ICC's) from eyes of adult newts (__Notophthalmus viridescens__) are cultured on top of pituitary glands they produce advanced lens regenerates in up to 90% of the cases (this report and Connelly et al., '73). To determine if the iris and pituitary gland mus

Role of retinoic acid in lens regenerati
Role of retinoic acid in lens regeneration
✍ Panagiotis A. Tsonis; Michael T. Trombley; Timothy Rowland; Roshantha A.S. Chand πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 404 KB
Inhibition of lens regeneration in larva
Inhibition of lens regeneration in larvalXenopus laevis
✍ Cioni, Carla ;Filoni, Sergio ;Bosco, Luigi πŸ“‚ Article πŸ“… 1982 πŸ› John Wiley and Sons 🌐 English βš– 900 KB

## Abstract The present research aims at showing the role played by the lens in inhibiting lens‐forming transformations of the outer cornea of __Xenopus laevis__ tadpoles. Two types of experiment were carried out: 1) lentectomy and insertion of a Millipore filter disk at the side of the lens, and 2

Increase in mitotic activity of regenera
Increase in mitotic activity of regenerating axolotl limbs by growth factor-impregnated implants
✍ Chew, Keith E. ;Cameron, Jo Ann πŸ“‚ Article πŸ“… 1983 πŸ› John Wiley and Sons 🌐 English βš– 469 KB

This study was designed to investigate the effects of pituitary fibroblast growth factor (FGF), endothelial cell growth supplement (ECGS), and human serum albumin (HSA) on the mitotic indices of dedifferentiating stump cells in regenerating limbs deprived of a wound epidermis. These cells would norm