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Control of macromolecular synthesis in proliferating and resting syrian hamster cells in monolayer culture. I. Ribosome function

✍ Scribed by C. P. Stanners; H. Becker

Publisher
John Wiley and Sons
Year
1971
Tongue
English
Weight
989 KB
Volume
77
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

The rate of protein synthesis per cell in cultured hamster embryo fibroblasts in the stationary growth phase falls to about one third of the rate in the exponential growth phase. This reduction can be entirely accounted for by the following observations: (1) the average cell in stationary phase contains about one‐half the number of ribosomes per cell compared to the average cell in exponential phase; (2) only two thirds of the ribosomes are bound to polysomes in stationary phase, while nearly all of the ribosomes are polysome‐bound in exponential phase.

In stationary phase, ribosomes which are polysome‐bound function with the same efficiency and produce proteins of approximately the same average length as in exponential phase. Experimental findings are presented which suggest that the generation of a higher proportion of free ribosomes in stationary phase in not due to a limitation in messenger RNA, but to a decreased attachment probability of ribosomes to messenger RNA.

📜 SIMILAR VOLUMES

Control of macromolecular synthesis in p
Control of macromolecular synthesis in proliferating and resting syrian hamster cells in monolayer culture. II. Ribosome complement in resting and early G1 Cells
✍ H. Becker; C. P. Stanners; J. E. Kudlow 📂 Article 📅 1971 🏛 John Wiley and Sons 🌐 English ⚖ 624 KB

## Abstract Using a new, sensitive and quantitative technique for determining the ribosomal‐RNA content of a measured number of cells, the cellular ribosome complement was compared for cultured hamster embryo cells in the stationary growth phase and in the early G~1~ phase of the cell cycle. Cells

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Control of macromolecular synthesis in proliferating and resting syrian hamster cells in monolayer culture. III. Electrophoretic patterns of newly synthesized proteins in synchronized proliferating cells and resting cells
✍ H. Becker; C. P. Stanners 📂 Article 📅 1972 🏛 John Wiley and Sons 🌐 English ⚖ 887 KB

## Abstract Electrophoretic patterns of newly synthesized proteins have been compared for hamster embryo fibroblasts in asynchronous cultures, mitotically synchronized cultures, and stationary phase cultures. Only proteins with molecular weight between 30,000 and 150,000, comprising 60–70% of the t