Computer and calculator programs have been prepared which can predict sedimentation times for a significant fraction of the kinds of density gradient centrifugation experiments currently being carried out in biochemistry. Gradients of sucrose, glycerol, or C&l were accommodated for linear or several
Continuous sedimentation theory: Effects of density gradients and velocity profiles on sedimentation efficiency
β Scribed by T.W.M. Wouda; K. Rietema; S.P.P. Ottengraf
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 750 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0009-2509
No coin nor oath required. For personal study only.
β¦ Synopsis
A theory IS presented on contmuous sedunentation In case the sohds concentration LS small and umformly &striiuted over the mlet he&t, the theory predxts mdependent sedunentabon e5clencles on velocity Qstnbutions m a longttudmal vertxal plane A velocity profile m a horuontal plane on the other hand wdl have a negative effect on the efficiency Experunents, camed out on a laboratory-scale model, have shown that even small density dfiereuces m the basm can have a s@cant effect on the velocity Qstnbution The measured e5clencles are m good agreement with the theory 1 mTR0Ducrl~
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